Immunocytochemistry analysis of GATA3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in GATA3 Monoclonal antibody (Product # MA5-45049) using a dilution of 1:100 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 Goat anti-Mouse IgG secondary antibody at a dilution of 1:100. The nuclear counter stain is DAPI (blue).